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TECHNOLOGIES

Ezose Sciences Glycan Analysis Capabilities

Addressing Bottlenecks in Glycomics

GlycanMap® Platform

As a pioneer in glycomics and glycan analysis methodologies, Ezose Sciences offers our partners access to a proprietary, high-throughput solution that is compatible with complex biological samples and can deliver both qualitative and quantitative data on N-linked and O-linked glycans. The GlycanMap® platform, Ezose’s proprietary core technology, combines a bead-based separation technology and fully-automated sample processing with high-throughput MALDI mass spectrometry and robust bioinformatics capabilities to generate rapid, reproducible glycan structural profiles.

glycan analysis process Click to Enlarge

The three key components of the GlycanMap® assay include:
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Glycoblotting

Ezose Sciences uses glycoblotting technology, which is faster and easier than conventional methods. This rapid chemoselective process features an “all-in-one” bead-based platform technology that enriches oligosaccharides from crude mixtures, enabling highly selective and high-throughput purification of oligosaccharides from biological samples such as serum, CSF, tissue or cell lysates, and cell culture media. Results are proven comparable to standard lower-throughput methods.

Automated Processing

Our customized 96-well robotics system provides the throughput and repeatability required for large-scale glycomics studies, facilitating analysis of hundreds of samples for process development and robust biomarker discovery.

Proprietary Quantitative Mass Spectrometry and Bioinformatics

Ezose Sciences employs a unique and proprietary protocol that, in combination with MALDI mass spectrometry, enables glycan identification and quantitation with reproducibility comparable to HPLC, yet at a far greater throughput. High-resolution mass spectrometry provides accurate glycan identification, with sumultaneous detection of both neutral and acidic oligosaccharides, including sialic acids. Proprietary bioinformatics provide absolute quantitation by comparing peak intensities to those of internal standards.

In addition to our core GlycanMap® technology, Ezose Sciences also has a full suite of assay development and glycan and protein characterization capabilities, including LC, LC/MS, MSn, glycopeptide analysis, and protein analysis.

Your Next Step

Our team will be happy to discuss your individual research needs and to provide additional details about working with Ezose.
Contact Ezose Sciences today to get started.



ENABLING DISCOVERY THROUGH OUR NEXT-GENERATION GLYCAN ANALYTICS PLATFORM


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